Praktikum Mikrobiologi

 

Module title SBEP3051 Microbiology Laboratory
Semester(s) 5th Semester
Person responsible Dr. Samingan, M.Si.
Language Indonesian, English
Relation to curriculum Compulsory modules for 5th semester students. This laboratory course provides practical reinforcement to the theoretical foundations covered in Microbiology. It supports CPL-6 and CPL-7 by engaging students in techniques such as microbial staining, culture preparation, aseptic technique, and microbial observation under a microscope. In line with ASIIN’s focus on professional and scientific competencies, the course cultivates students’ technical accuracy, hygiene discipline, and analytical thinking. It also strengthens students’ understanding of microbial diversity, structure, and roles in ecosystems and human contexts.
Teaching methods Contextual, group discussion, discovery learning, lecture based on field observation, inquiry approach, video project, practical report
Workload 1 x 45 hours per semester, comprising:
✔ 170 minutes structured tasks per week
Credit points 1 SKS = 1.6 ECTS (45 hours)
Prerequisites –
Module objectives ✔ Students are able to operate microbiological equipment, prepare media, sterilize tools and materials, and perform cultivation and purification techniques.
✔ Students are able to design and conduct laboratory activities and prepare reports on MPN tests, microbial analysis, staining techniques, and fermentation processes.
Content This course is conducted through laboratory activities focusing on microbiological techniques.

Main topics:
➢ Introduction to microbiology laboratory tools and materials
➢ Preparation of microbial growth media
➢ Sterilization techniques
➢ Cultivation, enumeration, and purification of microbial isolates
➢ MPN method for coliform detection
➢ Examination of soil and airborne microorganisms
➢ Bacterial staining techniques
➢ Observation of fungal and yeast morphology
➢ Fermentation techniques using fruit juice
➢ Enzymatic reactions of microorganisms
Exams and assessment formats Quiz, assignments, team-based project (report, video, presentation, Q&A)
Assessment Project Results: 50%
Assignment: 25%
Quiz: 25%
Recommended Literature 1. Prescott, L. M., & Harley, J. P. (2002). Laboratory exercises in microbiology (5th ed.). McGraw-Hill Companies.
2. Hogg, S. (2005). Essential microbiology. John Wiley & Sons Ltd.
3. Ristiati, N. (2000). Pengantar mikrobiologi umum. Proyek Pengembangan Guru Sekolah Menengah, Direktorat Jenderal Pendidikan Tinggi, Departemen Pendidikan Nasional.
4. Hogg, S. (2005). Essential microbiology. John Wiley & Sons, Ltd.
5. Brock, T. D., & Madigan, M. T. (1991). Biology of microorganisms. Prentice Hall.
6. Talaro, K. P., & Talaro, A. (2002). Foundations in microbiology (4th ed.). McGraw-Hill Education.
7. Tortora, G. J., Funke, B. R., & Case, C. L. (2010). Microbiology: An introduction (10th ed.). Benjamin Cummings.
8. Shimasaki, C. (Ed.). (2020). Biotechnology entrepreneurship: Leading, managing and commercializing innovative technologies (2nd ed.). Academic Press.
9. Tortora, G. J., Funke, B. R., & Case, C. L. (2018). Microbiology: An introduction (13th ed.). Pearson.
10. Cowan, M. K., & Smith, H. (2023). Microbiology: A Systems Approach (7th ed., International Student Edition). McGraw-Hill Education.
11. Mangieri, N., Arioli, S., Isotti, M., Russo, P., & Mora, D. (2026). Exploring food spoilage: An inquiry-based laboratory for food microbiology education.
12. Made Easy Academy. (2025). Simply Said Science: Microbiology Full Course Study Guide: Key Topics in Bacteria, Viruses, Fungi, Immunology, and Disease Pathogenesis (in Terms You’ll Actually Understand). Made Easy Academy.
13. Kowalczyk, M., Domaradzki, P., Stanek, P., Ziomek, M., Daszkiewicz, T., Chmielowiec-Korzeniowska, A., Kędzierska-Matysek, M., Grenda, T., Ryszkowska-Siwko, M., & Florek, M. (2026). Effect of frozen storage period and temperature on the physicochemical and microbiological quality of veal. Meat Science, 240, 110141. https://doi.org/10.1016/j.meatsci.2026.110141
Date of Amendment August 2024